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Get Price- What is polyacrylamide gel electrophoresis?
- Different separation media and mechanisms allow subsets of these molecules to be separated more effectively by exploiting their physical characteristics. For proteins in particular, polyacrylamide gel electrophoresis (PAGE) is often the technique of choice. What is polyacrylamide gel electrophoresis and what is protein electrophoresis?
- Can online polyacrylamide gel electrophoresis be used for nucleic acid detection?
- We developed an online polyacrylamide gel electrophoresis platform for nucleic acid detection. The online platform avoided the complicated offline procedure and band broadening. We also achieved the real-time monitoring of DNA separation during the gel electrophoresis.
- Can gel electrophoresis detect high-resolution DNA separation?
- We also achieved the real-time monitoring of DNA separation during the gel electrophoresis. A 32–64 % increase in the number of theoretical plates suggested high-resolution DNA detection. Polyacrylamide gel electrophoresis (PAGE) is one of the most popular techniques for the separation and detection of nucleic acids.
- Why is polyacrylamide better than agarose?
- The pores formed in polyacrylamide are smaller than those of agarose, used for agarose gel electrophoresis. This makes it more suitable for the separation of proteins over large polynucleotide DNA or RNA fragments and allows the separation of relatively small proteins.
