Biofouling of Polymer Hydrogel Materials and its Effect
Biofouling of Polymer Hydrogel Materials and its Effect on Diffusion and Enzyme-Based Luminescent Glucose Sensor Functional Characteristics Show all authors. Jason R. Roberts 1. D. Diffusion of glucose and maltose in polyacrylamide gel.
Diffusion in polyacrylamide gels W. Brown and R. M. Johnsen Institute of Physical Chemistry, University of Uppsala, Box 532, S-751 21 Uppsala, Sweden (Received 1 5 January 1 980; revised 24 July 1980) Using a novel sorption technique, the diffusion of some series of solutes in polyacrylamide gels has been investigated with regard to: (a) molecular size of solute; (b) concentration of solute
Simultaneous intraparticle diffusion, reaction and aerobic
The diffusion of glucose and maltose in different polyacrylamide gels (PAAG) was investigated. The method applied was non-steady-state diffusion into gel beads from a finite solution.
Get Price
Transport of Glucose and Poly(ethylene glycol)s in Agarose
The change in glucose concentration in agarose gel with the diffusion distance and the diffusion kinetics of glucose in agarose gels reveal that glucose diffuses freely within these gel matrixes with a high diffusion coefficient 鈭? 脳 10 -10 m 2 /s for 0.5% and 1.5% agarose gels.
Get Price
Modelling of enzyme hydrolysis of maltose in a single
The reversible hydrolysis of maltose to glucose by immobilized glucoamylase entrapped in spherical solid particles is studied theoretically. For this purpose a known kinetic model taking into account these reversible reactions and the competitive synthesis of iso-maltose was adopted.
Get Price
Specificity ofdiffusionchannels Xphage Escherichia coli
specificity for maltose and maltodextrins observed in intact cells, it has been proposed that the lamB protein produces a totally nonspecificchannel, andthat thespecificity is derived entirely from the interaction of periplasmic maltose-binding protein with the lamBprotein (7). Toexamine the function of the lamB protein in vitro, we
Get Price
Maltose metabolism of Pseudomonas fluorescens. - Europe
Pseudomonas fluorescens W uses maltose exclusively by hydrolyzing it to glucose via an inducible alpha-glucosidase (alpha-D-glucoside glucohydrolase, EC 3.2.1.20). No evidence for phosphorolytic cleavage or oxidation to maltobionic acid was found in this organism. The alpha-glucosidase was totally intracellular and was most active at pH of 7.0.
Get Price
Production of Antimicrobial Metabolites by Bacillus
subtilis immobilized in polyacrylamide gel and screened for the production of antibiotics by shake flask fermentation at 30oC by checking activity against Micrococcus luteus (ATCC#10240) through antibiotics diffusion assay. Maximum production of peptide antibiotic was optimized at pH 6-9, incubation time 0-144 hours and glucose concentration 1-5 %.
Get Price
Laws of the Formation and Diffusion Properties of Silica
The physicochemical and mass-transfer properties were studied for two gels that can be applied in the fabrication of bioreactors using additive manufacturing. The fundamental difference between these gels lies in the mechanisms of their formation from solutions. The first one is a silicic acid gel, which is formed in a chemical reaction and has an irreversible gelling process.
Get Price
Dialysis with icodextrin interferes with measurement
The reaction mixture was incubated for 16 h at 37掳C and then air dried for 2 h. Samples were dissolved in electrophoresis sample buffers and then polyacrylamide gel electrophoreses was performed (ExcelGel TM SDS, Homogeneous 12,5, Amersham Pharmacia Biotech, Uppsala Sweden, 600 V, 30 mA, 7 h). Gels were photographed under UV light (360 nm).
Get Price
Glucose- and Glucokinase-Controlled mal Gene Expression
The Escherichia coli maltose system (4, 52) is geared for the efficient utilization of maltose and maltodextrins.Ten mal genes encode proteins found in all compartments of the cell. The lambda receptor in the outer membrane (43, 49) facilitates the diffusion of maltodextrins into the periplasmic space, where they are taken up into the cytoplasm via a binding-protein-dependent ABC transporter
Get Price
Modelling of enzyme hydrolysis of maltose in a single
The reversible hydrolysis of maltose to glucose by immobilized glucoamylase entrapped in spherical solid particles is studied theoretically. For this purpose a known kinetic model taking into account these reversible reactions and the competitive synthesis of iso-maltose was adopted.
Get Price
Moderate Concentrations of Ethanol Inhibit Endocytosis
acrylamide gel electrophoresis SDS-PAGE (10 and 8% polyacrylamide, respec-tively) (22), with antiserum against the maltose transporter or against ATPase diluted 1/3,000 and 1/15,000, respectively, and goat anti-rabbit peroxidase con-jugate diluted 1/10,000 in blocking buffer as the primary and secondary antibod-ies.
Get Price
Isolation Characterization aBacillus cereus Mutant Strain
addition of glucose led to a decrease in the P-amylase production, as was the case with its parental strains (Fig. 1A). Amylase production was also observed to increase to 5,400 U/ml after the addition of 1% maltose to the basal medium. In contrast, the amylase activities of strains BQ10-S1andBQ10-S1 SpoIIwerenotenhancedbymaltose (Fig. 1B).
Get Price
CORE
(1998). Diffusion of chromium ions in polymer/silicate gels. (2004). Diffusion of glucose and maltose in polyacrylamide gel. (2004). Diffusion of H+, H2O and D2O in polymer/silicate gels. (1984). Diffusion: Mass Transfer in Fluid Systems, (2006).
Get Price
IJESRT
value of test (maltose formed by the action of 伪-amylase on starch) was similar to standard maltose. Native polyacrylamide gel electrophoresis (PAGE) was conducted to determine the homogeneity of 伪-amylase at pH 8.3 in 12% slab gel. Specific staining was conducted to confirm the protein band as 伪-amylase.
Get Price
Request quick quotes from Parchem - chemical supplier
COVID-19 STATUS: Parchem is open and ready to supply materials 1-800-282-3982 READ MORE
Get Price
Planta 148, 354-361 (1980) Planta - JSTOR
and preparative disc gel electrophoresis. The two en zymes were homogeneous by Polyacrylamide disc gel electrophoresis. Their molecular weights were 98,000 (I) and 60,000 (II). a-Glucosidase I readily hydrolyzed maltose, isomaltose, kojibiose, maltotriose, panose, amylose, soluble starch, amylopectin and glycogen.
Get Price
Microfabricated Polyacrylamide Devices for the Controlled
The ability to spatially confine living cells or small organisms while dynamically controlling their aqueous environment is important for a host of microscopy applications. Here, we show how polyacrylamide layers can be patterned to construct simple microfluidic devices for this purpose. We find that polyacrylamide gels can be molded like PDMS into micron-scale structures that can enclose
Get Price
IJESRT
value of test (maltose formed by the action of 伪-amylase on starch) was similar to standard maltose. Native polyacrylamide gel electrophoresis (PAGE) was conducted to determine the homogeneity of 伪-amylase at pH 8.3 in 12% slab gel. Specific staining was conducted to confirm the protein band as 伪-amylase.
Get PricePurification and Characterization of Extracellular Acidic
Gel staining Gel was removed from glass plates and stained overnight with staining solution (2.5% Coomassie Brilliant Blue containing methanol and acetic acid in the ratio of 40:7). The excess stain was removed by diffusion in destaining solution (7.5 % acetic acid and 5.0% methanol). After complete destaining, gel was transferred to
Get Price
Characterization of dextran芒 producing Leuconostoc strains
(70 ll) into stacking-gel sample wells. Electrophoresis was performed in a 12脝5% resolving gel at room temperature at a current of 20 mA. In situ detection of sucrases Following SDS-PAGE, the gel was washed three times in sodium acetate buffer (20 mmol l)1 sodium acetate, pH 5脝4, 0脝3 mmol l)1 CaCl and 0脝1% Tween 80) at 4掳C for a total time
Get Price
Reactivity-mapping studies on biopolymers in hydrogel
(1998). Diffusion of chromium ions in polymer/silicate gels. (2004). Diffusion of glucose and maltose in polyacrylamide gel. (2004). Diffusion of H+, H2O and D2O in polymer/silicate gels. (1984). Diffusion: Mass Transfer in Fluid Systems, (2006).
Get Price- Does anionic polyacrylamide biodegrade?
- ent only.1 BACKGROUNDAnionic polyacrylamide is the copolymer of acryl mide and acrylic acid. No studies on the environmental fate of polyac ylamide are available. As a high-molecular weight, water-soluble polymer, it is not expected to biode rade or bioaccumulate. Anionic polyacrylamide has a low acute toxicity concer
- Which comonomer is used for the production of anionic polyacrylamides?
- The workhorse comonomer for the production of anionic polyacrylamides is acrylate salts of acrylic acid. These anionic polymers can be prepared as various mono- and divalent metal salts and other positively charged inorganic and organic counterions of the polymerization of acrylic acid with acrylamide (see Fig. 34.4 ).
- What type of polyacrylamide is used in oil industry?
- The polyacrylamide use can be anionic, cationic, or nonionic with various ratios of the comonomers used in the case of the anionic and cationic polymers. The anionic polyacrylamides in the oil field industry are designated by the generic name of partially hydrolyzed polyacrylamide (PHPA), although they are in actuality copolymers .
- Is polyacrylamide a cationic or nonionic polymer?
- rade or bioaccumulate. Anionic polyacrylamide has a low acute toxicity concer CO-CHEMICAL PROPERTIESPolyacrylamide polymers can exist in cationic, anionic or non-ionic forms, depending on their ionic charge. The non-ionic form of polyacrylamide is generated from the basic polyme
